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taqman preamplification kit #4384267  (Thermo Fisher)


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    Thermo Fisher taqman preamplification kit #4384267
    Taqman Preamplification Kit #4384267, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/preamplification+kit/taqman+preamplification+kit++4384267/us11945881-3064-18-21
    Average 90 stars, based on 1 article reviews
    taqman preamplification kit #4384267 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Expression and Characterization of a Murine Enzyme Able to Cleave β-Carotene
    Article Snippet: .. Characterization of Carotene 15,15 -Dioxygenase Expression by RTPCR—Total RNA (3 g) from liver, testes, intestine, and kidney (for isolation details, see above) was first treated with DNase I to remove any genomic DNA contamination, and reverse transcription (RT) was performed using a preamplification kit (Life Technologies, Inc.) and random hexamers according to the manufacturer’s recommendations. ..

    Article Title: Genetic dissection of a major mouse obesity QTL ( Carfhg2 ): integration of gene expression and causality modeling
    Article Snippet: High-throughput real-time quantitative PCR using BioMark Dynamic Arrays. .. The expression of a total of 48 genes (44 target genes and four endogenous controls) was measured in adipose, whole brain, liver, and muscle using ABI Taqman Gene Expression Assays (ABI) (Supplemental Table S2) and BioMark 48.48 Dynamic Arrays (Fluidigm, South San Francisco, CA) ( 20 ). cDNA was preamplified using the ABI PreAmplification Kit. .. Preamplified cDNA was loaded into the Dynamic Array along with 2× Master Mix (ABI) and Loading Agent (Fluidigm).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Expression and Characterization of a Murine Enzyme Able to Cleave β-Carotene
    Article Snippet: .. Characterization of Carotene 15,15 -Dioxygenase Expression by RTPCR—Total RNA (3 g) from liver, testes, intestine, and kidney (for isolation details, see above) was first treated with DNase I to remove any genomic DNA contamination, and reverse transcription (RT) was performed using a preamplification kit (Life Technologies, Inc.) and random hexamers according to the manufacturer’s recommendations. ..

    Isolation:

    Article Title: Expression and Characterization of a Murine Enzyme Able to Cleave β-Carotene
    Article Snippet: .. Characterization of Carotene 15,15 -Dioxygenase Expression by RTPCR—Total RNA (3 g) from liver, testes, intestine, and kidney (for isolation details, see above) was first treated with DNase I to remove any genomic DNA contamination, and reverse transcription (RT) was performed using a preamplification kit (Life Technologies, Inc.) and random hexamers according to the manufacturer’s recommendations. ..

    Reverse Transcription:

    Article Title: Expression and Characterization of a Murine Enzyme Able to Cleave β-Carotene
    Article Snippet: .. Characterization of Carotene 15,15 -Dioxygenase Expression by RTPCR—Total RNA (3 g) from liver, testes, intestine, and kidney (for isolation details, see above) was first treated with DNase I to remove any genomic DNA contamination, and reverse transcription (RT) was performed using a preamplification kit (Life Technologies, Inc.) and random hexamers according to the manufacturer’s recommendations. ..

    Article Title: Tissue-Specific Transgenic Knockdown of Fos-Related Antigen 2 (Fra-2) Expression Mediated by Dominant Negative Fra-2
    Article Snippet: .. Full-length cDNAs in plasmid vectors (2 μg) were denatured, immobilized onto a nylon membrane (Hybond N + ; Amersham-Pharmacia) using a slot blot apparatus (Schleicher & Schuell, Keene, N.H.), and hybridized with 32 P-radiolabeled cDNAs reverse transcribed from total pineal RNA samples (1 μg) using a preamplification kit (Life Technologies). ..

    Article Title: Evaluation of current methods used to analyze the expression profiles of ABC transporters yields an improved drug-discovery database
    Article Snippet: .. 1.25 μl of reverse transcribed cDNA from each sample was preamplified with the use of the Applied Biosystems PreAmplification Kit. ..

    Gene Expression:

    Article Title: Genetic dissection of a major mouse obesity QTL ( Carfhg2 ): integration of gene expression and causality modeling
    Article Snippet: High-throughput real-time quantitative PCR using BioMark Dynamic Arrays. .. The expression of a total of 48 genes (44 target genes and four endogenous controls) was measured in adipose, whole brain, liver, and muscle using ABI Taqman Gene Expression Assays (ABI) (Supplemental Table S2) and BioMark 48.48 Dynamic Arrays (Fluidigm, South San Francisco, CA) ( 20 ). cDNA was preamplified using the ABI PreAmplification Kit. .. Preamplified cDNA was loaded into the Dynamic Array along with 2× Master Mix (ABI) and Loading Agent (Fluidigm).

    cDNA Synthesis:

    Article Title: Protein Kinase Cδ Activation by Interleukin-1β Stabilizes Inducible Nitric-oxide Synthase mRNA in Pancreatic β-Cells
    Article Snippet: Cells were then washed in PBS and resuspended in 1 ml of Trizol/well (Life Technologies, Inc.). .. RNA was extracted according to the manufacturer’s instructions and quantified using absorbance at 260 nm. cDNA synthesis was performed using a preamplification kit (Life Technologies, Inc.) according to the manufacturer’s instructions, using 5 mg of RNA in each reaction. .. PCR was then performed using 2 ml of cDNA, oligonucleotides specific for either b-actin or iNOS (39), and AmpliTaq Gold (PerkinElmer Life Sciences).

    Article Title: The role of the renin-angiotensin system in cholesterol and puromycin mediated renal injury.
    Article Snippet: Background: Puromycin aminonucleoside (PAN) nephropathy is a widely studied model of glomerular sclerosis (GS) in the rat, and cholesterol feeding exacerbates the injury induced by PAN.. The importance of the interaction of angiotensin II (Ang II) with the AT2 receptor is unclear.. We investigated the role of the renin-angiotensin system, particularly with regard to AT1 and AT2 receptor dynamics, in PAN and cholesterolmediated GS.

    Plasmid Preparation:

    Article Title: Tissue-Specific Transgenic Knockdown of Fos-Related Antigen 2 (Fra-2) Expression Mediated by Dominant Negative Fra-2
    Article Snippet: .. Full-length cDNAs in plasmid vectors (2 μg) were denatured, immobilized onto a nylon membrane (Hybond N + ; Amersham-Pharmacia) using a slot blot apparatus (Schleicher & Schuell, Keene, N.H.), and hybridized with 32 P-radiolabeled cDNAs reverse transcribed from total pineal RNA samples (1 μg) using a preamplification kit (Life Technologies). ..

    Membrane:

    Article Title: Tissue-Specific Transgenic Knockdown of Fos-Related Antigen 2 (Fra-2) Expression Mediated by Dominant Negative Fra-2
    Article Snippet: .. Full-length cDNAs in plasmid vectors (2 μg) were denatured, immobilized onto a nylon membrane (Hybond N + ; Amersham-Pharmacia) using a slot blot apparatus (Schleicher & Schuell, Keene, N.H.), and hybridized with 32 P-radiolabeled cDNAs reverse transcribed from total pineal RNA samples (1 μg) using a preamplification kit (Life Technologies). ..

    Dot Blot:

    Article Title: Tissue-Specific Transgenic Knockdown of Fos-Related Antigen 2 (Fra-2) Expression Mediated by Dominant Negative Fra-2
    Article Snippet: .. Full-length cDNAs in plasmid vectors (2 μg) were denatured, immobilized onto a nylon membrane (Hybond N + ; Amersham-Pharmacia) using a slot blot apparatus (Schleicher & Schuell, Keene, N.H.), and hybridized with 32 P-radiolabeled cDNAs reverse transcribed from total pineal RNA samples (1 μg) using a preamplification kit (Life Technologies). ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Immunohistochemical application of a highly sensitive and specific murine monoclonal antibody recognising the extracellular domain of the human hepatocyte growth factor receptor (MET).
    Article Snippet: One lg of total RNA and RNA to cDNA EcoDryTM Premix Double Primed (Clontech, Mountain View, CA, USA) were used for reverse transcription reaction according to manufacturer’s instructions. .. Total RNA was extracted (100 ng) from FFPE lines, and ABI High Capacity RNA to cDNA kit followed by target specific preamplification with ABI Preamplification Kit (Life Technologies, Grand Island, NY, USA) was used for cDNA conversion according to manufacturer instructions. ..



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